Denmark evaluation

The target is to test Sterinis^® effects  in a patient room, contaminated with known micro-organisms. The disinfection effect was controlled by putting sampling germs on areas, then after to put these samples for growth analysis.

Trial design:
A patient room (59m3) currently not in use at intensive ward 4131, was used for the trials. All medical apparatus were kept with in addition a respiratory device. 20 sample sites were selected and contaminated with a mixture of Serratia marcescens, Enterococcus faecalis og Bacillus cereus, all in a concentration of  10⁷-10⁸ CFU/ml. Sterinis® was programmed for 3  diffusion cycles of 14 minutes with contact times respectively of 30 minutes, 1 hour and 2 hours. After the disinfection, the samples were taken and put in identified petri-boxes with blood-agar for growth then the samples were put in a serum treatment during 48hours at 37°C for incubation

Results and comments:

2 samples were not sterile, these results are foreseeable and due to the extreme difficulty to reach these sample sites (This result was expected and worked like a ”positive control”):

                  +++ 50 cm inside of a tube-hose (rubber-tube)

                  +++ inside of a suction-glass with a sealed top

Sample place	S. marcescens	E. faecalis	B. cereus
On top of cupboard beside door	0	0	0
On top of cupboard beside window	0	0	0
Ceiling lamp by door	0	0	0
Ceiling lamp by window	0	0	0
Soap-dispenser holder -wall	0	0	0
Screw holes in wall	0	0	0
Thermostat on heater-wall	0	0	0
Cotton curtain	0	0	0
Under table by window	0	0	0
Computer keyboard	0	0	0
Respiratory hose entry	0	0	0
Respiratory hose exit	0	0	0
Respiratory hose middle	+++	+++	0
Respirations mask	0	0	0
Infusionpump	0	0	0
Under monitor	0	0	0
Apparatus display	0	0	0
Top of monitor box	0	0	0
Suction bottle	0	+++	+++

Prospects: Where can Sterinis^® be used at the Rikshospital:

In the following places and situations Sterinis^® can be used:

•  In wards or sections with out-brakes of micro-organisms without finding the source.
• In sections where there are many cases of certain types of infections (fx Clostridium difficile).
• In high-risk areas, like intensive care, neonatal ward, transplantation sections.
• In selected cases of end-disinfection of isolation wards after infected patients.
• During the re-building etc... when spores might be present.
• For disinfection of technical apparatus in high-risk areas.
• For disinfection of ambulances.

Comments:

Disinfection with Sterinis® does not last longer than traditional methods, but the disinfection performance is safer and more secure, especially for areas not easily reachable..


 Evaluation report from Rigshospitalet : Full evaluation report